survival and activity of lux-marked phenanthrene-degrading pseudomonas stutzeri p16 under different conditions

نویسندگان

m mashreghi

چکیده

viable cell concentration and luminescence activity of p. stutzeri p16 luxab4 was measured byplate counting and luminometery in sterile and non-sterile soil adjusted to matric potential of -1500, -750 and-30 kpa. results showed that matric potential had a significant effect on survival and activity of p. stutzerip16 luxab4 in soil. p. stutzeri p16 luxab4 could survive better in hutner’s minimal medium supplementedwith 1mg l-1 phenanthrene than 1 mg ml-1. however changes in viable cell concentration and luminescenceactivity of lux-marked and wild type strains of p. stutzeri p16 were significantly different in hutner’smedium, amended with high and low concentrations of phenanthrene. inoculation of soil with highconcentrations of phenanthrene caused an initial decline in the viable cell concentration of p. stutzeri p16luxab4 that took a longer time than liquid medium. changes in viable cell concentration were similar tochanges in luminescence indicating the advantage of bioluminescence-marker systems for monitoring thesurvival and activity of biodegradative genetically engineered microorganisms (gems) in pollutedenvironments. our results also showed the importance of influence of environmental factors such as matricpotential and pollutant concentration on the fate of gems.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Survival and Activity of lux-Marked Aeromonas salmonicida in Seawater.

The fish pathogen Aeromonas salmonicida was chromosomally marked with genes encoding bacterial luciferase, luxAB, isolated from Vibrio fischeri, resulting in constitutive luciferase production. During exponential growth in liquid batch culture, luminescence was directly proportional to biomass concentration, and luminometry provided a lower detection limit of approximately 10(sup3) cells ml(sup...

متن کامل

Siderophore production by Pseudomonas stutzeri under aerobic and anaerobic conditions.

The siderophore production of the facultative anaerobe Pseudomonas stutzeri, strain CCUG 36651, grown under both aerobic and anaerobic conditions, was investigated by liquid chromatography and mass spectrometry. The bacterial strain has been isolated at a 626-m depth at the Aspö Hard Rock Laboratory, where experiments concerning the geological disposal of nuclear waste are performed. In bacteri...

متن کامل

Survival and Metabolic Characteristics of Lux-Marked Escherichia Coli O157:H7 in Different Types of Milk

Escherichia coli O157:H7 is a potentially lethal pathogen which has been responsible for several outbreaks of milk-borne illness in recent years. The objective of this study was to evaluate the survival and metabolic activity (indexed by bioluminescence) of a chromosomally lux-marked strain of E. coli O157:H7 in raw, pasteurized and microfiltered pasteurized milk at 4 and 20°C for up to 14 d. R...

متن کامل

Effect of model sorptive phases on phenanthrene biodegradation: different enrichment conditions influence bioavailability and selection of phenanthrene-degrading isolates.

The sorption of organic contaminants by natural organic matter (NOM) often limits substrate bioavailability and is an important factor affecting microbial degradation rates in soils and sediments. We hypothesized that reduced substrate bioavailability might influence which microbial assemblages are responsible for contaminant degradation under enrichment culture conditions. Our primary goal was...

متن کامل

Repression of Pseudomonas putida phenanthrene-degrading activity by plant root extracts and exudates.

The phenanthrene-degrading activity (PDA) of Pseudomonas putida ATCC 17484 was repressed after incubation with plant root extracts of oat (Avena sativa), osage orange (Maclura pomifera), hybrid willow (Salix alba x matsudana), kou (Cordia subcordata) and milo (Thespesia populnea) and plant root exudates of oat (Avena sativa) and hybrid poplar (Populus deltoides x nigra DN34). Total organic carb...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of science and technology (sciences)

ISSN 1028-6276

دوره 30

شماره 1 2006

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023